Andrographis paniculata is a herb well known in Asia and belongs to the family Acanthaceae. It is native to Asia and distributed throughout India, China, Sri Lanka and Taiwan. Andrographis paniculata is a potential medicinal plant of immense economic importance. Leaves of A. paniculata contains active constitutents like andrographolide, homoandrographolide, andrographestrol and andrographonone. A. paniculata, also known as Kalmegh in India, is mentioned in ancient Indian medicine (Ayurvedic) literature as a constituent of ayurvedic formulation used for the treatment of liver disorder, and neoplasm. The plant has also hepatoprotective, antioxidant, and hypoglycemic activities and is being used in the management of immune system and neoplastic diseases.

Due to limited information with regard to genomic resources, essential for exploitation of Andrographis paniculata, we have generated a database from its entire transcriptome including information relevant to gene sequences and gene-based SSR (EST-SSR) or microsatellite markers.   SSR marker is an indespensible tool for molecular mapping, diversity analysis and phylogenetic studies. Moreover, since SSR were designed from transcripts sequences, it is equally useful for comparative genomics including candidate gene determination. Besides molecular markers, the sequences of transcripts will serves as an important resource for the discovery of biosynthetic genes and other regulons associated with its active constituents.

Citation: Singh, R.; Singh, A.; Mahato, A.K.; Paliwal, R.; Tiwari, G.; Kumar, A. De Novo Transcriptome Profiling for generation and validation of Microsatellite Markers and Transcription Factors in Andrographis paniculata (“Kalmegh”) and development of Database. International Journal of Molecular Sciences 2023, 24, 9212.