1) Krait v1.3.3
2) Primer3 V1.0
Mono= (1/10)
Di-nucleotide= (2/6)
Tri-nucleotide=(3/5)
Tetra-nucleotide= (4/5)
Penta-nucleotide= (5/5)
Hexa-nucleotide =(6/5)
1
2. Annealing temperature=650C; GC content of (40-60%) with optimum of 50%;
3. Remaining parameters were kept at the default setting for Primer3 V1.0
Results: